Journal: Journal of the Turkish German Gynecological Association

Article Title: ARID4B loss leads to activated STAT1-dependent interferon pathway in mouse embryonic stem cells and during meso/endodermal differentiation.

doi: 10.4274/jtgga.galenos.2023.2023-7-5

Figure Lengend Snippet: Figure 4. ARID4B loss leads to increased STAT1 level and activation at the mESC stage and during endoderm differentiation. (a) Western blot of ARID4B, STAT1, pSTAT1 (Tyr701) and loading control ACTIN using wild-type and arid4b∆ cell lysates during endoderm differentiation time-course, (b) Western blot of STAT3, pSTAT3 (Tyr705) and loading control ACTIN using wild-type and arid4b∆ cell lysates during endoderm differentiation time-course. Day 0 of differentiation represents the mESC stage mESC: Mouse embryonic stem cell

Article Snippet: Antibodies used were: ARID4B (Bethyl Laboratories, A302-233A), ACTIN (Millipore, MAB1501), STAT1 (CST, 9172), pSTAT1 (CST, 9167), STAT3 (CST, 9139), pSTAT3 (CST, 9145).

Techniques: Activation Assay, Western Blot, Control

Journal: Nature Communications

Article Title: Structural studies of the IFNλ4 receptor complex using cryoEM enabled by protein engineering

doi: 10.1038/s41467-025-56119-y

Figure Lengend Snippet: A pSTAT1 signaling of IFNω1 (orange), IFNλ3 (blue), and IFNλ4 (red) in Hap1 cells. Curves are fit to a first-order logistic model. Data are presented with mean values and error bars representing ±SEM ( n = 3 biologically independent experiments). B Relative quantification (RQ) of select genes induced by IFNω1, IFNλ3, and IFNλ4 in Hap1 cells treated with saturating concentrations (100 nM) interferon for 6 h as measured by qPCR. Data are presented with mean values. Error bars represent 95% confidence intervals ( n = 3 biologically independent experiments). C Changes in induction of ISG15 or MX1 over time for IFNλ3 and IFNλ4 in Huh7.5.1 cells at a range of concentrations (~0.5, 1.5, 5, and 15 nM). Statistical significance determined by a two-tailed student t -test. Data are presented as mean values ± SD ( n = 3 biologically independent experiments; * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001). D Intracellular HCV genomic RNA level over time following treatment with IFNω1, IFNλ3, or IFNλ4 in Huh7.5.1 cells. Statistical significance was determined by a two-tailed student t -test. Data are presented as mean values ± SD ( n = 3 biologically independent experiments; * = p ≤ 0.05, ** = p ≤ 0.01).

Article Snippet: To quantify activation of pSTAT1, cells were washed thrice with 0.5% PBSA and then stained with an anti-Y701 pSTAT1 monoclonal antibody (Cell Signaling, product #9174S) according to manufacturer instructions.

Techniques: Quantitative Proteomics, Two Tailed Test